i sdf 1α solution Search Results


125 i-sdf-1α  (DuPont de Nemours)
90
DuPont de Nemours 125 i-sdf-1α
Binding of X4 gp120s directly to CXCR4. The X4 gp120s from BH8 and MN were iodinated and used for binding to 4 × 105 transfected 293T cells expressing CXCR4. All conditions contained 100 nM sCD4 except where indicated. Values represent the average and standard error of two to three independent experiments. To best represent the signal-to-noise levels achieved in this assay, only background binding to filters alone was subtracted from values. Raw values of binding and background binding to cells not expressing CXCR4 are presented in Fig. ​Fig.6.6. BH8 exhibited high background binding in the presence of cells regardless of blocking or transfection conditions, and thus the minimal binding of BH8 in the presence of cells was 30% of total binding. Blocking agents and concentrations were as follows: JRFL gp120 (R5), MN gp120 (X4), and BH8 gp120 (X4) Envs (250 to 500 nM); 12G5 (anti-CXCR4), D47 (BH8-specific anti-V3 loop), and mIgG (pooled mouse IgG) MAbs (10 μg/ml); IL-8 (CXCR2 ligand) and <t>SDF-1α</t> (CXCR4 ligand) chemokines (100 nM); EDTA (10 mM); and ALX40-4C (anti-CXCR4 antagonist) (5 to 10 μM).
125 I Sdf 1α, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i+sdf+1%CE%B1+solution/pmc00104032-71-4-9?v=DuPont+de+Nemours
Average 90 stars, based on 1 article reviews
125 i-sdf-1α - by Bioz Stars, 2026-07
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Binding of X4 gp120s directly to CXCR4. The X4 gp120s from BH8 and MN were iodinated and used for binding to 4 × 105 transfected 293T cells expressing CXCR4. All conditions contained 100 nM sCD4 except where indicated. Values represent the average and standard error of two to three independent experiments. To best represent the signal-to-noise levels achieved in this assay, only background binding to filters alone was subtracted from values. Raw values of binding and background binding to cells not expressing CXCR4 are presented in Fig. ​Fig.6.6. BH8 exhibited high background binding in the presence of cells regardless of blocking or transfection conditions, and thus the minimal binding of BH8 in the presence of cells was 30% of total binding. Blocking agents and concentrations were as follows: JRFL gp120 (R5), MN gp120 (X4), and BH8 gp120 (X4) Envs (250 to 500 nM); 12G5 (anti-CXCR4), D47 (BH8-specific anti-V3 loop), and mIgG (pooled mouse IgG) MAbs (10 μg/ml); IL-8 (CXCR2 ligand) and SDF-1α (CXCR4 ligand) chemokines (100 nM); EDTA (10 mM); and ALX40-4C (anti-CXCR4 antagonist) (5 to 10 μM).

Journal:

Article Title: Identification of CXCR4 Domains That Support Coreceptor and Chemokine Receptor Functions

doi:

Figure Lengend Snippet: Binding of X4 gp120s directly to CXCR4. The X4 gp120s from BH8 and MN were iodinated and used for binding to 4 × 105 transfected 293T cells expressing CXCR4. All conditions contained 100 nM sCD4 except where indicated. Values represent the average and standard error of two to three independent experiments. To best represent the signal-to-noise levels achieved in this assay, only background binding to filters alone was subtracted from values. Raw values of binding and background binding to cells not expressing CXCR4 are presented in Fig. ​Fig.6.6. BH8 exhibited high background binding in the presence of cells regardless of blocking or transfection conditions, and thus the minimal binding of BH8 in the presence of cells was 30% of total binding. Blocking agents and concentrations were as follows: JRFL gp120 (R5), MN gp120 (X4), and BH8 gp120 (X4) Envs (250 to 500 nM); 12G5 (anti-CXCR4), D47 (BH8-specific anti-V3 loop), and mIgG (pooled mouse IgG) MAbs (10 μg/ml); IL-8 (CXCR2 ligand) and SDF-1α (CXCR4 ligand) chemokines (100 nM); EDTA (10 mM); and ALX40-4C (anti-CXCR4 antagonist) (5 to 10 μM).

Article Snippet: Subsequently, 0.1 nM 125 I-SDF-1α (specific activity, 2,200 Ci/mmol; NEN-Dupont) was added to cells in 25 μl of binding buffer for a total volume of 100 μl.

Techniques: Binding Assay, Transfection, Expressing, Blocking Assay